Acetylcholine release from dissociated striatal cells

Ivan S. Login, Kate Borland, Madaline B. Harrison, Michael E. Ragozzino, Paul E. Gold

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


To study the regulation of striatal acetylcholinee (ACH) release, adult male rat striata were dissociated and incubated with 3H-choline to synthesize 3H-ACH. Fractional 3H-ACH efflux per min during continuous perifussion was: (1) tightly regulated; (2) dependent on calcium influx; (3) stimulated by 10 mM K+ and 1 mM glutamate; and (4) comparable to ACH release detected by HPLC. Thus, acutely dissociated striata exhibit calcium-sensitive, voltage-dependent secretion of 3H-ACH and direct receptor-mediated stimulation of release through the glutamate receptor family. This new approach toward cholinergic secretory physiology will help clarify complex striatal circuitry.

Original languageEnglish (US)
Pages (from-to)271-275
Number of pages5
JournalBrain Research
Issue number1-2
StatePublished - Oct 30 1995
Externally publishedYes


  • Acetylcholine
  • Calcium
  • Dissociated cell
  • Fractional efflux
  • Glutamate
  • High-performance liquid chromatography
  • Striatum

ASJC Scopus subject areas

  • General Neuroscience
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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