Acetylcholine release from dissociated striatal cells

Ivan S. Login, Kate Borland, Madaline B. Harrison, Michael E. Ragozzino, Paul Ernest Gold

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To study the regulation of striatal acetylcholinee (ACH) release, adult male rat striata were dissociated and incubated with 3H-choline to synthesize 3H-ACH. Fractional 3H-ACH efflux per min during continuous perifussion was: (1) tightly regulated; (2) dependent on calcium influx; (3) stimulated by 10 mM K+ and 1 mM glutamate; and (4) comparable to ACH release detected by HPLC. Thus, acutely dissociated striata exhibit calcium-sensitive, voltage-dependent secretion of 3H-ACH and direct receptor-mediated stimulation of release through the glutamate receptor family. This new approach toward cholinergic secretory physiology will help clarify complex striatal circuitry.

Original languageEnglish (US)
Pages (from-to)271-275
Number of pages5
JournalBrain Research
Volume697
Issue number1-2
DOIs
StatePublished - Oct 30 1995
Externally publishedYes

Fingerprint

Corpus Striatum
Acetylcholine
Calcium
Glutamate Receptors
Choline
Cholinergic Agents
Glutamic Acid
High Pressure Liquid Chromatography

Keywords

  • Acetylcholine
  • Calcium
  • Dissociated cell
  • Fractional efflux
  • Glutamate
  • High-performance liquid chromatography
  • Striatum

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Login, I. S., Borland, K., Harrison, M. B., Ragozzino, M. E., & Gold, P. E. (1995). Acetylcholine release from dissociated striatal cells. Brain Research, 697(1-2), 271-275. https://doi.org/10.1016/0006-8993(95)00996-4

Acetylcholine release from dissociated striatal cells. / Login, Ivan S.; Borland, Kate; Harrison, Madaline B.; Ragozzino, Michael E.; Gold, Paul Ernest.

In: Brain Research, Vol. 697, No. 1-2, 30.10.1995, p. 271-275.

Research output: Contribution to journalArticle

Login, IS, Borland, K, Harrison, MB, Ragozzino, ME & Gold, PE 1995, 'Acetylcholine release from dissociated striatal cells', Brain Research, vol. 697, no. 1-2, pp. 271-275. https://doi.org/10.1016/0006-8993(95)00996-4
Login IS, Borland K, Harrison MB, Ragozzino ME, Gold PE. Acetylcholine release from dissociated striatal cells. Brain Research. 1995 Oct 30;697(1-2):271-275. https://doi.org/10.1016/0006-8993(95)00996-4
Login, Ivan S. ; Borland, Kate ; Harrison, Madaline B. ; Ragozzino, Michael E. ; Gold, Paul Ernest. / Acetylcholine release from dissociated striatal cells. In: Brain Research. 1995 ; Vol. 697, No. 1-2. pp. 271-275.
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