Acetylcholine release from dissociated striatal cells

Ivan S. Login; Kate Borland; Madaline B. Harrison; Michael E. Ragozzino; Paul E. Gold

doi:10.1016/0006-8993(95)00996-4

Acetylcholine release from dissociated striatal cells

Ivan S. Login, Kate Borland, Madaline B. Harrison, Michael E. Ragozzino, Paul E. Gold

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

To study the regulation of striatal acetylcholinee (ACH) release, adult male rat striata were dissociated and incubated with 3H-choline to synthesize 3H-ACH. Fractional 3H-ACH efflux per min during continuous perifussion was: (1) tightly regulated; (2) dependent on calcium influx; (3) stimulated by 10 mM K⁺ and 1 mM glutamate; and (4) comparable to ACH release detected by HPLC. Thus, acutely dissociated striata exhibit calcium-sensitive, voltage-dependent secretion of 3H-ACH and direct receptor-mediated stimulation of release through the glutamate receptor family. This new approach toward cholinergic secretory physiology will help clarify complex striatal circuitry.

Original language	English (US)
Pages (from-to)	271-275
Number of pages	5
Journal	Brain Research
Volume	697
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(95)00996-4
State	Published - Oct 30 1995
Externally published	Yes

Keywords

Acetylcholine
Calcium
Dissociated cell
Fractional efflux
Glutamate
High-performance liquid chromatography
Striatum

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(95)00996-4

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title = "Acetylcholine release from dissociated striatal cells",

abstract = "To study the regulation of striatal acetylcholinee (ACH) release, adult male rat striata were dissociated and incubated with 3H-choline to synthesize 3H-ACH. Fractional 3H-ACH efflux per min during continuous perifussion was: (1) tightly regulated; (2) dependent on calcium influx; (3) stimulated by 10 mM K+ and 1 mM glutamate; and (4) comparable to ACH release detected by HPLC. Thus, acutely dissociated striata exhibit calcium-sensitive, voltage-dependent secretion of 3H-ACH and direct receptor-mediated stimulation of release through the glutamate receptor family. This new approach toward cholinergic secretory physiology will help clarify complex striatal circuitry.",

keywords = "Acetylcholine, Calcium, Dissociated cell, Fractional efflux, Glutamate, High-performance liquid chromatography, Striatum",

author = "Login, {Ivan S.} and Kate Borland and Harrison, {Madaline B.} and Ragozzino, {Michael E.} and Gold, {Paul E.}",

note = "Funding Information: This work was supported by NIH Research Grants AG 07648, CA 38228, NS 30024, NS 32914 and the American Parkinson Disease Association.",

year = "1995",

month = oct,

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doi = "10.1016/0006-8993(95)00996-4",

language = "English (US)",

volume = "697",

pages = "271--275",

journal = "Brain Research",

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TY - JOUR

T1 - Acetylcholine release from dissociated striatal cells

AU - Login, Ivan S.

AU - Borland, Kate

AU - Harrison, Madaline B.

AU - Ragozzino, Michael E.

AU - Gold, Paul E.

N1 - Funding Information: This work was supported by NIH Research Grants AG 07648, CA 38228, NS 30024, NS 32914 and the American Parkinson Disease Association.

PY - 1995/10/30

Y1 - 1995/10/30

N2 - To study the regulation of striatal acetylcholinee (ACH) release, adult male rat striata were dissociated and incubated with 3H-choline to synthesize 3H-ACH. Fractional 3H-ACH efflux per min during continuous perifussion was: (1) tightly regulated; (2) dependent on calcium influx; (3) stimulated by 10 mM K+ and 1 mM glutamate; and (4) comparable to ACH release detected by HPLC. Thus, acutely dissociated striata exhibit calcium-sensitive, voltage-dependent secretion of 3H-ACH and direct receptor-mediated stimulation of release through the glutamate receptor family. This new approach toward cholinergic secretory physiology will help clarify complex striatal circuitry.

AB - To study the regulation of striatal acetylcholinee (ACH) release, adult male rat striata were dissociated and incubated with 3H-choline to synthesize 3H-ACH. Fractional 3H-ACH efflux per min during continuous perifussion was: (1) tightly regulated; (2) dependent on calcium influx; (3) stimulated by 10 mM K+ and 1 mM glutamate; and (4) comparable to ACH release detected by HPLC. Thus, acutely dissociated striata exhibit calcium-sensitive, voltage-dependent secretion of 3H-ACH and direct receptor-mediated stimulation of release through the glutamate receptor family. This new approach toward cholinergic secretory physiology will help clarify complex striatal circuitry.

KW - Acetylcholine

KW - Calcium

KW - Dissociated cell

KW - Fractional efflux

KW - Glutamate

KW - High-performance liquid chromatography

KW - Striatum

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ER -

Acetylcholine release from dissociated striatal cells

Abstract

Keywords

ASJC Scopus subject areas

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