Accumulation of potentiometric and other dyes in haustoria of Erysiphe graminis in living host cell

W. R. Bushnell, K. Mendgen, Zhanjian "John" Liu

Research output: Contribution to journalArticle

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Abstract

Twenty-five vital fluorescent dyes were tested for accumulation in haustoria of Erysiphe graminis (DC) Merat f. sp. hordei Em. Marchal when applied to coleoptile epidermal host cells of Hordeum vulgare L. Eight of the dyes gave fluorescence to structures judged to be haustorial mitochondria. The potentiometric cyanine dyes, DiOC4(3) and DiOC7(3) gave best differentiation between the putative mitochondria and other structures. As measured photometrically, fluorescence of the organelles diminished within a few minutes after DNP(1 × 10-4m), CCCP (1 × 10-6m), or KCN (1 × 10-3m) was applied to host tissue, indicating that fluorescence depended on the membrane potential of the organelles. Many of the tested dyes, including several cyanines, accumulated in haustorial cytoplasm (excluding mitochondria or vacuoles), but the uptake of most of these dyes was not affected by DNP or CCCP, indicating that the dyes moved passively into haustoria. However the uptake of the cyanines DiOC5(3) and DiSC2(3) was slightly reduced by CCCP suggesting that a component of uptake of these dyes depended on the potential of the plasmalemma or extrahaustorial membrane of the haustorium. The cyanine potentiometric dyes provide a way to monitor electrical potential across mitochondrial and other membranes which, in turn, may relate to functional competence of haustoria.

Original languageEnglish (US)
Pages (from-to)237-250
Number of pages14
JournalPhysiological and Molecular Plant Pathology
Volume31
Issue number2
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Blumeria graminis
dyes
Coloring Agents
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Mitochondria
Fluorescence
cells
mitochondria
Organelles
fluorescence
uptake mechanisms
organelles
Cotyledon
Mitochondrial Membranes
Hordeum
Vacuoles
Fluorescent Dyes
fluorescent dyes
Membrane Potentials
Mental Competency

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Cite this

Accumulation of potentiometric and other dyes in haustoria of Erysiphe graminis in living host cell. / Bushnell, W. R.; Mendgen, K.; Liu, Zhanjian "John".

In: Physiological and Molecular Plant Pathology, Vol. 31, No. 2, 1987, p. 237-250.

Research output: Contribution to journalArticle

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