A yeast gene (BEM1) necessary for cell polarization whose product contains two SH3 domains

Janet Chenevert, Kathleen Corrado, Alan Bender, John Pringle, Ira Herskowitz

Research output: Contribution to journalArticlepeer-review

180 Scopus citations


CELL polarization requires that a cellular axis or cell-surface site be chosen and that the cytoskeleton be organized with respect to it. Details of the link between the cytoskeleton and the chosen axis or site are not clear1. Cells of the yeast Saccharomyces cerevisiae exhibit cell polarization in two phases of their life cycle, during vegetative growth and during mating, which reflects responses to intracellular and extracellular signals, respectively. Here we describe the isolation of two mutants defective specifically in cell polarization in response to peptide mating pheromones. The mutants carry special alleles (denoted bem1-s) of the BEM1 gene required for cell polarization during vegetative growth2,3. Unlike other bem1 mutants, the bem1-s mutants are normal for vegetative growth. Complete deletion of BEM1 leads to the defect in polarization of vegetative cells seen in bem1 mutants2,3. The predicted sequence of the BEM1 protein (Bern1p) reveals two copies of a domain (denoted SH3) that is found in many proteins associated with the cortical cytoskeleton and which may mediate binding to actin or some other component of the cell cortex4,5. The sequence of Bem1p and the properties of mutants defective in this protein indicate that it may link the cytoskeleton to morphogenetic determinants on the cell surface.

Original languageEnglish (US)
Pages (from-to)77-79
Number of pages3
Issue number6364
StatePublished - Mar 5 1992
Externally publishedYes

ASJC Scopus subject areas

  • General


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