A water soluble vitamin B12-Re(I) fluorescent conjugate for cell uptake screens: Use in the confirmation of cubilin in the lung cancer line A549

Anthony R. Vortherms, Anna R. Kahkoska, Amy E. Rabideau, Jon Zubieta, Louise Lund Andersen, Mette Madsen, Robert P. Doyle

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

A water soluble vitamin B12-rhenium conjugate was synthesized and used in concert with intrinsic factor to screen for cubilin receptor-mediated uptake in lung cancer cells. Internalization of the conjugate demonstrated that it could be used to rapidly screen for the cubilin receptor in living cells, subsequently confirmed with Western blotting and RT-PCR.

Original languageEnglish (US)
Pages (from-to)9792-9794
Number of pages3
JournalChemical Communications
Volume47
Issue number35
DOIs
StatePublished - Sep 21 2011

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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