A water soluble vitamin B12-Re(I) fluorescent conjugate for cell uptake screens: Use in the confirmation of cubilin in the lung cancer line A549

Anthony R. Vortherms; Anna R. Kahkoska; Amy E. Rabideau; Jon Zubieta; Louise Lund Andersen; Mette Madsen; Robert P. Doyle

doi:10.1039/c1cc13615a

A water soluble vitamin B₁₂-Re(I) fluorescent conjugate for cell uptake screens: Use in the confirmation of cubilin in the lung cancer line A549

Anthony R. Vortherms, Anna R. Kahkoska, Amy E. Rabideau, Jon Zubieta, Louise Lund Andersen, Mette Madsen, Robert P. Doyle

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40 Scopus citations

Abstract

A water soluble vitamin B₁₂-rhenium conjugate was synthesized and used in concert with intrinsic factor to screen for cubilin receptor-mediated uptake in lung cancer cells. Internalization of the conjugate demonstrated that it could be used to rapidly screen for the cubilin receptor in living cells, subsequently confirmed with Western blotting and RT-PCR.

Original language	English (US)
Pages (from-to)	9792-9794
Number of pages	3
Journal	Chemical Communications
Volume	47
Issue number	35
DOIs	https://doi.org/10.1039/c1cc13615a
State	Published - Sep 21 2011

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
General Chemistry
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Catalysis

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abstract = "A water soluble vitamin B12-rhenium conjugate was synthesized and used in concert with intrinsic factor to screen for cubilin receptor-mediated uptake in lung cancer cells. Internalization of the conjugate demonstrated that it could be used to rapidly screen for the cubilin receptor in living cells, subsequently confirmed with Western blotting and RT-PCR.",

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AU - Rabideau, Amy E.

AU - Zubieta, Jon

AU - Andersen, Louise Lund

AU - Madsen, Mette

AU - Doyle, Robert P.

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AB - A water soluble vitamin B12-rhenium conjugate was synthesized and used in concert with intrinsic factor to screen for cubilin receptor-mediated uptake in lung cancer cells. Internalization of the conjugate demonstrated that it could be used to rapidly screen for the cubilin receptor in living cells, subsequently confirmed with Western blotting and RT-PCR.

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A water soluble vitamin B₁₂-Re(I) fluorescent conjugate for cell uptake screens: Use in the confirmation of cubilin in the lung cancer line A549

Abstract

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