A water soluble vitamin B 12-Re(I) fluorescent conjugate for cell uptake screens: Use in the confirmation of cubilin in the lung cancer line A549

Anthony R. Vortherms, Anna R. Kahkoska, Amy E. Rabideau, Jon A Zubieta, Louise Lund Andersen, Mette Madsen, Robert Patrick Doyle

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A water soluble vitamin B 12-rhenium conjugate was synthesized and used in concert with intrinsic factor to screen for cubilin receptor-mediated uptake in lung cancer cells. Internalization of the conjugate demonstrated that it could be used to rapidly screen for the cubilin receptor in living cells, subsequently confirmed with Western blotting and RT-PCR.

Original languageEnglish (US)
Pages (from-to)9792-9794
Number of pages3
JournalChemical Communications
Volume47
Issue number35
DOIs
StatePublished - Sep 21 2011

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Vitamins
Vitamin B 12
Cells
Rhenium
Intrinsic Factor
Water
intrinsic factor-cobalamin receptor

ASJC Scopus subject areas

  • Metals and Alloys
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Catalysis
  • Chemistry(all)

Cite this

A water soluble vitamin B 12-Re(I) fluorescent conjugate for cell uptake screens : Use in the confirmation of cubilin in the lung cancer line A549. / Vortherms, Anthony R.; Kahkoska, Anna R.; Rabideau, Amy E.; Zubieta, Jon A; Andersen, Louise Lund; Madsen, Mette; Doyle, Robert Patrick.

In: Chemical Communications, Vol. 47, No. 35, 21.09.2011, p. 9792-9794.

Research output: Contribution to journalArticle

Vortherms, Anthony R. ; Kahkoska, Anna R. ; Rabideau, Amy E. ; Zubieta, Jon A ; Andersen, Louise Lund ; Madsen, Mette ; Doyle, Robert Patrick. / A water soluble vitamin B 12-Re(I) fluorescent conjugate for cell uptake screens : Use in the confirmation of cubilin in the lung cancer line A549. In: Chemical Communications. 2011 ; Vol. 47, No. 35. pp. 9792-9794.
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