TY - GEN
T1 - A study of protein filaments sliding through solid-state nanopores
AU - McMullen, Angus
AU - Mihovilovic, Mirna
AU - Stein, Derek
AU - Tang, Jay X.
PY - 2011
Y1 - 2011
N2 - Solid-state nanopores have been used extensively over the past decade as powerful tools to study the structure and dynamics of single molecules of DNA. The use of nanopores has mostly been confined to the study of DNA due to the hope that they may eventually be used to cheaply sequence DNA. We have begun extending nanopores to the study of protein filaments, specifically filamentous actin (F-actin), one of three major components of the cytoskeleton. F-actin is a linear, negatively charged, helical polymer well suited for use in a nanopore setup. F-actin, however, behaves qualitatively different in solution than DNA as a result of their differences in persistence length. This enforces a different set of constraints on an F-actin molecule's interaction with a nanopore, allowing for new insights into the polymer dynamics of translocation through a nanometer-sized pore.
AB - Solid-state nanopores have been used extensively over the past decade as powerful tools to study the structure and dynamics of single molecules of DNA. The use of nanopores has mostly been confined to the study of DNA due to the hope that they may eventually be used to cheaply sequence DNA. We have begun extending nanopores to the study of protein filaments, specifically filamentous actin (F-actin), one of three major components of the cytoskeleton. F-actin is a linear, negatively charged, helical polymer well suited for use in a nanopore setup. F-actin, however, behaves qualitatively different in solution than DNA as a result of their differences in persistence length. This enforces a different set of constraints on an F-actin molecule's interaction with a nanopore, allowing for new insights into the polymer dynamics of translocation through a nanometer-sized pore.
KW - Actin
KW - Bio-sensing
KW - Polymer physics
KW - Single-molecule study
KW - Solid-state nanopores
UR - http://www.scopus.com/inward/record.url?scp=81455139962&partnerID=8YFLogxK
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M3 - Conference contribution
AN - SCOPUS:81455139962
SN - 9781439871386
T3 - Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
SP - 113
EP - 116
BT - Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
T2 - Nanotechnology 2011: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
Y2 - 13 June 2011 through 16 June 2011
ER -