A study of biologically active peptide sequences (P-15) on the surface of an ABM scaffold (PepGen P-15™) using AFM and FTIR

Bhushan B. Hole, James A. Schwarz, Jeremy L. Gilbert, Brent L. Atkinson

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Cellular response to any biomaterial surface is governed by a number of factors including topography, surface chemistry, surface charge, structural heterogeneity, and physiological conditions. Understanding these factors at the nanoscale level is crucial to develop improved biomaterials. Any changes in these properties due to surface modifications need to be addressed properly, as they could have significant impact on the cellular interaction with biomaterials. In this study, the topography and surface chemistry of commercially available tissue engineered xenograft, PepGen P-15™ [comprised of a synthetic peptide P-15 irreversibly attached to anorganic bovine bone mineral (OsteoGraf/-N®)] was studied using Atomic Force Microscopy (AFM), and Fourier Transform Infrared Spectroscopy (FTIR). FTIR confirmed the presence of the peptide on the surface of PepGen P-15™. Changes in the peptide conformation, which includes a decrease in the β-strand accompanied by an increase in unordered structures/random coil structures after attachment on OsteoGraf/-N® is observed. Specific functional groups, which are involved in the binding mechanism, are identified. The results suggest that the attachment of the peptide on OsteoGraf/-N® occurs via a specific surface docking ionic interaction involving the C-terminal carboxylic group on the peptide with positive domains generated by hydroxyl vacancies on the apatite surface.

Original languageEnglish (US)
Pages (from-to)712-721
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume74
Issue number4
DOIs
StatePublished - Sep 15 2005

Keywords

  • AFM
  • FTIR
  • Hydroxyapatite
  • Tissue engineering
  • Xenograft

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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