A spectroscopic investigation of the metal binding site of bleomycin A2. The Cu(II) and Zn(II) derivatives

James C. Dabrowiak, Frederick T. Greenaway, Walter E. Longo, Mark Van Husen, Stanley T. Crooke

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

Using a combination of ultraviolet-visible absorption, 1H NMR and ESR techniques we have established that N(1) of the imidazole and N(1) of the pyrimidine residues of bleomycin A2 bind to Cu(II) and Zn(II). These observations coupled with the earlier results that the α-amino group of the α-amino carboxamide function and the carbamoyl moiety are also Cu(II)-ligating groups makes it possible to reconstruct the detailed geometry and stereochemistry of the metal binding site of bleomycin A2.

Original languageEnglish (US)
Pages (from-to)517-526
Number of pages10
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume517
Issue number2
DOIs
StatePublished - Feb 16 1978

ASJC Scopus subject areas

  • Medicine(all)

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