A spectroscopic investigation of the metal binding site of bleomycin A2. The Cu(II) and Zn(II) derivatives

James C. Dabrowiak, Frederick T. Greenaway, Walter E. Longo, Mark Van Husen, Stanley T. Crooke

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Abstract

Using a combination of ultraviolet-visible absorption, 1H NMR and ESR techniques we have established that N(1) of the imidazole and N(1) of the pyrimidine residues of bleomycin A2 bind to Cu(II) and Zn(II). These observations coupled with the earlier results that the α-amino group of the α-amino carboxamide function and the carbamoyl moiety are also Cu(II)-ligating groups makes it possible to reconstruct the detailed geometry and stereochemistry of the metal binding site of bleomycin A2.

Original languageEnglish (US)
Pages (from-to)517-526
Number of pages10
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume517
Issue number2
DOIs
StatePublished - Feb 16 1978

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Bleomycin
Metals
Binding Sites
Proton Magnetic Resonance Spectroscopy
pyrimidine
imidazole

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A spectroscopic investigation of the metal binding site of bleomycin A2. The Cu(II) and Zn(II) derivatives. / Dabrowiak, James C.; Greenaway, Frederick T.; Longo, Walter E.; Van Husen, Mark; Crooke, Stanley T.

In: BBA Section Nucleic Acids And Protein Synthesis, Vol. 517, No. 2, 16.02.1978, p. 517-526.

Research output: Contribution to journalArticle

Dabrowiak, James C. ; Greenaway, Frederick T. ; Longo, Walter E. ; Van Husen, Mark ; Crooke, Stanley T. / A spectroscopic investigation of the metal binding site of bleomycin A2. The Cu(II) and Zn(II) derivatives. In: BBA Section Nucleic Acids And Protein Synthesis. 1978 ; Vol. 517, No. 2. pp. 517-526.
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