A rhodopsin is the functional photoreceptor for phototaxis in the unicellular eukaryote Chlamydomonas

K. W. Foster, J. Saranak, N. Patel, G. Zarilli, M. Okabe, T. Kline, K. Nakanishi

Research output: Contribution to journalArticle

258 Citations (Scopus)

Abstract

Rhodopsin is a visual pigment ubiquitous in multicellular animals. If visual pigments have a common ancient origin, as is believed, then some unicellular organisms might also use a rhodopsin photoreceptor. We show here that the unicellular alga Chlamydomonas does indeed use a rhodopsin photoreceptor. We incorporated analogues of its retinal chromophore into a blind mutant; normal photobehaviour was restored and the colour of maximum sensitivity was shifted in a manner consistent with the nature of the retinal analogue added. The data suggest that 11-cis-retinal is the natural chromophore and that the protein environment of this retinal is similar to that found in bovine rhodopsin, suggesting homology with the rhodopsins of higher organisms. This is the first demonstration of a rhodopsin photoreceptor in an alga or eukaryotic protist and also the first report of behavioural spectral shifts caused by exogenous synthetic retinals in a eukaryote. A survey of the morphology and action spectra of other protists suggests that rhodopsins may be common photoreceptors of chlorophycean, prasinophycean and dinophycean algae. Thus, Chlamydomonas represents a useful new model for studying photoreceptor cells.

Original languageEnglish (US)
Pages (from-to)756-759
Number of pages4
JournalNature
Volume311
Issue number5988
DOIs
StatePublished - 1984

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Chlamydomonas
Rhodopsin
Eukaryota
Retinal Pigments
Retinaldehyde
Photoreceptor Cells
Phototaxis
Color

ASJC Scopus subject areas

  • General

Cite this

Foster, K. W., Saranak, J., Patel, N., Zarilli, G., Okabe, M., Kline, T., & Nakanishi, K. (1984). A rhodopsin is the functional photoreceptor for phototaxis in the unicellular eukaryote Chlamydomonas. Nature, 311(5988), 756-759. https://doi.org/10.1038/311756a0

A rhodopsin is the functional photoreceptor for phototaxis in the unicellular eukaryote Chlamydomonas. / Foster, K. W.; Saranak, J.; Patel, N.; Zarilli, G.; Okabe, M.; Kline, T.; Nakanishi, K.

In: Nature, Vol. 311, No. 5988, 1984, p. 756-759.

Research output: Contribution to journalArticle

Foster, KW, Saranak, J, Patel, N, Zarilli, G, Okabe, M, Kline, T & Nakanishi, K 1984, 'A rhodopsin is the functional photoreceptor for phototaxis in the unicellular eukaryote Chlamydomonas', Nature, vol. 311, no. 5988, pp. 756-759. https://doi.org/10.1038/311756a0
Foster KW, Saranak J, Patel N, Zarilli G, Okabe M, Kline T et al. A rhodopsin is the functional photoreceptor for phototaxis in the unicellular eukaryote Chlamydomonas. Nature. 1984;311(5988):756-759. https://doi.org/10.1038/311756a0
Foster, K. W. ; Saranak, J. ; Patel, N. ; Zarilli, G. ; Okabe, M. ; Kline, T. ; Nakanishi, K. / A rhodopsin is the functional photoreceptor for phototaxis in the unicellular eukaryote Chlamydomonas. In: Nature. 1984 ; Vol. 311, No. 5988. pp. 756-759.
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