This short note presents a recipe for the calculation of the ionic permeabilities across epithelial cell membranes. The method requires the Goldman-Hodgkin-Katz formalism as well as the consideration of the equivalent electrical circuit for an epithelial cell. The equivalent electrical circuit is solved in terms of the equivalent electromotive forces coupled in series with the ionic resistances of both cell membranes (apical and basolateral). The present procedure is feasible for any leaky epithelial cell membrane with the condition that this membrane (apical or basolateral) does not contain primary or secondary mechanisms for active transport.
- Cell model
- Epithelial cell membranes
- Equivalent electrical circuit
- Goldman-Hodgkin-Katz formalism
- Intracellular micro-electrode technique
- Ionic permeabilities
ASJC Scopus subject areas