A rapid method for the evaluation of the ionic permeabilities across epithelial cell membranes

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1 Scopus citations

Abstract

This short note presents a recipe for the calculation of the ionic permeabilities across epithelial cell membranes. The method requires the Goldman-Hodgkin-Katz formalism as well as the consideration of the equivalent electrical circuit for an epithelial cell. The equivalent electrical circuit is solved in terms of the equivalent electromotive forces coupled in series with the ionic resistances of both cell membranes (apical and basolateral). The present procedure is feasible for any leaky epithelial cell membrane with the condition that this membrane (apical or basolateral) does not contain primary or secondary mechanisms for active transport.

Original languageEnglish (US)
Pages (from-to)209-215
Number of pages7
JournalJournal of Biochemical and Biophysical Methods
Volume38
Issue number3
DOIs
StatePublished - Jan 29 1999
Externally publishedYes

Keywords

  • Cell model
  • Epithelial cell membranes
  • Equivalent electrical circuit
  • Goldman-Hodgkin-Katz formalism
  • Intracellular micro-electrode technique
  • Ionic permeabilities

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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