A programmable shape-changing scaffold for regenerative medicine

Ling Fang Tseng, Patrick T. Mather, James H. Henderson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

Conventional tissue engineering scaffolds have limited ability to undergo programmed changes in physical properties. Here we present a thermo-responsive and biocompatible tissue engineering scaffold prepared by electrospinning a shape memory polymer (SMP). SMPs have characteristics which allow them to be manipulated and fixed in a temporary shape and later recover back to their permanent shape on command. We hypothesized that a programmed change in scaffold architecture could control cell body orientation. To test this hypothesis, we uniaxially stretched an initially random mesh (the permanent state) and fixed it to a temporarily aligned mesh. After first seeding cells on the temporarily aligned mesh, we triggered a change in shape by increasing the temperature from 30°C to 37°C which resulted in the scaffold structure recovering back to its initial random structure. Alignment of cell bodies was quantified by two-dimensional fast Fourier transform (2D FFT) analysis of filamentous actin fibers. We found that before triggering a change in shape, cells aligned preferentially along the direction of fiber orientation. After the shape-memory-activated structure change, cells lost their preferential alignment. Shape-changing scaffolds based on this concept are anticipated to provide a powerful tool to study cell mechanobiology and increase tissue engineering scaffold functionality.

Original languageEnglish (US)
Title of host publication2012 38th Annual Northeast Bioengineering Conference, NEBEC 2012
Pages227-228
Number of pages2
DOIs
StatePublished - Jun 29 2012
Event38th Annual Northeast Bioengineering Conference, NEBEC 2012 - Philadelphia, PA, United States
Duration: Mar 16 2012Mar 18 2012

Publication series

Name2012 38th Annual Northeast Bioengineering Conference, NEBEC 2012

Other

Other38th Annual Northeast Bioengineering Conference, NEBEC 2012
CountryUnited States
CityPhiladelphia, PA
Period3/16/123/18/12

ASJC Scopus subject areas

  • Bioengineering

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    Tseng, L. F., Mather, P. T., & Henderson, J. H. (2012). A programmable shape-changing scaffold for regenerative medicine. In 2012 38th Annual Northeast Bioengineering Conference, NEBEC 2012 (pp. 227-228). [6207046] (2012 38th Annual Northeast Bioengineering Conference, NEBEC 2012). https://doi.org/10.1109/NEBC.2012.6207046