A micro device for measuring single-cell membrane specific capacitance and cytoplasm conductivity

Qingyuan Tan; Jian Chen; Yi Zheng; Brandon K. Chen; Yu Sun

doi:10.1109/MEMSYS.2012.6170296

A micro device for measuring single-cell membrane specific capacitance and cytoplasm conductivity

Qingyuan Tan, Jian Chen, Yi Zheng, Brandon K. Chen, Yu Sun

Research output: Chapter in Book/Entry/Poem › Conference contribution

3 Scopus citations

Abstract

This paper presents a micro device using micro electrical impedance spectroscopy (μEIS) to extract single-cell membrane specific capacitance and cytoplasm conductivity. Single cells were aspirated and stopped inside a wedge-shaped constriction channel where their impedance profiles were recorded by an impedance analyzer. An equivalent circuit model was developed to extract cell electrical parameters from the measured impedance profiles. The system was applied to quantify membrane specific capacitance (4.48±1.17 μF/cm ², n=11) and cytoplasm conductivity (0.59±0.33 S/m, n=11) of PAVICs (porcine aortic valve interstitial cells).

Original language	English (US)
Title of host publication	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Pages	757-760
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2012.6170296
State	Published - 2012
Externally published	Yes
Event	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France Duration: Jan 29 2012 → Feb 2 2012

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Conference

Conference	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Country/Territory	France
City	Paris
Period	1/29/12 → 2/2/12

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

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10.1109/MEMSYS.2012.6170296

Cite this

Tan, Q., Chen, J., Zheng, Y., Chen, B. K., & Sun, Y. (2012). A micro device for measuring single-cell membrane specific capacitance and cytoplasm conductivity. In 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 (pp. 757-760). Article 6170296 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2012.6170296

A micro device for measuring single-cell membrane specific capacitance and cytoplasm conductivity. / Tan, Qingyuan; Chen, Jian; Zheng, Yi et al.
2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012. 2012. p. 757-760 6170296 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Entry/Poem › Conference contribution

Tan, Q, Chen, J, Zheng, Y, Chen, BK & Sun, Y 2012, A micro device for measuring single-cell membrane specific capacitance and cytoplasm conductivity. in 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012., 6170296, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 757-760, 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012, Paris, France, 1/29/12. https://doi.org/10.1109/MEMSYS.2012.6170296

Tan Q, Chen J, Zheng Y, Chen BK, Sun Y. A micro device for measuring single-cell membrane specific capacitance and cytoplasm conductivity. In 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012. 2012. p. 757-760. 6170296. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2012.6170296

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title = "A micro device for measuring single-cell membrane specific capacitance and cytoplasm conductivity",

abstract = "This paper presents a micro device using micro electrical impedance spectroscopy (μEIS) to extract single-cell membrane specific capacitance and cytoplasm conductivity. Single cells were aspirated and stopped inside a wedge-shaped constriction channel where their impedance profiles were recorded by an impedance analyzer. An equivalent circuit model was developed to extract cell electrical parameters from the measured impedance profiles. The system was applied to quantify membrane specific capacitance (4.48±1.17 μF/cm 2, n=11) and cytoplasm conductivity (0.59±0.33 S/m, n=11) of PAVICs (porcine aortic valve interstitial cells).",

author = "Qingyuan Tan and Jian Chen and Yi Zheng and Chen, {Brandon K.} and Yu Sun",

year = "2012",

doi = "10.1109/MEMSYS.2012.6170296",

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N2 - This paper presents a micro device using micro electrical impedance spectroscopy (μEIS) to extract single-cell membrane specific capacitance and cytoplasm conductivity. Single cells were aspirated and stopped inside a wedge-shaped constriction channel where their impedance profiles were recorded by an impedance analyzer. An equivalent circuit model was developed to extract cell electrical parameters from the measured impedance profiles. The system was applied to quantify membrane specific capacitance (4.48±1.17 μF/cm 2, n=11) and cytoplasm conductivity (0.59±0.33 S/m, n=11) of PAVICs (porcine aortic valve interstitial cells).

AB - This paper presents a micro device using micro electrical impedance spectroscopy (μEIS) to extract single-cell membrane specific capacitance and cytoplasm conductivity. Single cells were aspirated and stopped inside a wedge-shaped constriction channel where their impedance profiles were recorded by an impedance analyzer. An equivalent circuit model was developed to extract cell electrical parameters from the measured impedance profiles. The system was applied to quantify membrane specific capacitance (4.48±1.17 μF/cm 2, n=11) and cytoplasm conductivity (0.59±0.33 S/m, n=11) of PAVICs (porcine aortic valve interstitial cells).

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T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

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A micro device for measuring single-cell membrane specific capacitance and cytoplasm conductivity

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