A micro device for impedance and mechanical characterization of biological cells

Jian Chen, Yi Zheng, Qingyuan Tan, Yan Liang Zhang, Jason Li, Steve To, Yu Sun

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper presents a microfluidic device for simultaneous electromechanical characterization of single cells. The device performs two types of cellular characterization (impedance spectroscopy and micropipette aspiration) on a single chip to enable cell electrical and mechanical characterization. To investigate the performance of the device design, electrical and mechanical properties of MC-3T3 osteoblast cells were measured. Based on electrical models, membrane capacitance of MC-3T3 cells was determined to be 3.39 ± 1.23 pF and 2.99 ± 0.82 pF at the aspiration pressure of 50 Pa and 100 Pa, respectively. Cytoplasm resistance values were 110.1±37.7 kω (50 Pa) and 145.2±44.3 kω (100 Pa). Aspiration length of cells was found to be 0.813± 0.351 m at 50 Pa and 1.771 ± 0.623 m at 100 Pa. Quantified Young's modulus values were 377±189 Pa at 50 Pa and 344±156 Pa at 100 Pa. Experimental results demonstrate the device's capability for characterizing both electrical and mechanical properties of single cells.

Original languageEnglish (US)
Title of host publication2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Pages1192-1195
Number of pages4
DOIs
StatePublished - 2011
Externally publishedYes
Event2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing, China
Duration: Jun 5 2011Jun 9 2011

Publication series

Name2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11

Conference

Conference2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Country/TerritoryChina
CityBeijing
Period6/5/116/9/11

Keywords

  • Cellular Biophysics
  • Impedance Spectroscopy
  • Microfluidics
  • Micropipette Aspiration
  • Single Cell Analysis

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

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