TY - GEN
T1 - A micro device for impedance and mechanical characterization of biological cells
AU - Chen, Jian
AU - Zheng, Yi
AU - Tan, Qingyuan
AU - Zhang, Yan Liang
AU - Li, Jason
AU - To, Steve
AU - Sun, Yu
PY - 2011
Y1 - 2011
N2 - This paper presents a microfluidic device for simultaneous electromechanical characterization of single cells. The device performs two types of cellular characterization (impedance spectroscopy and micropipette aspiration) on a single chip to enable cell electrical and mechanical characterization. To investigate the performance of the device design, electrical and mechanical properties of MC-3T3 osteoblast cells were measured. Based on electrical models, membrane capacitance of MC-3T3 cells was determined to be 3.39 ± 1.23 pF and 2.99 ± 0.82 pF at the aspiration pressure of 50 Pa and 100 Pa, respectively. Cytoplasm resistance values were 110.1±37.7 kω (50 Pa) and 145.2±44.3 kω (100 Pa). Aspiration length of cells was found to be 0.813± 0.351 m at 50 Pa and 1.771 ± 0.623 m at 100 Pa. Quantified Young's modulus values were 377±189 Pa at 50 Pa and 344±156 Pa at 100 Pa. Experimental results demonstrate the device's capability for characterizing both electrical and mechanical properties of single cells.
AB - This paper presents a microfluidic device for simultaneous electromechanical characterization of single cells. The device performs two types of cellular characterization (impedance spectroscopy and micropipette aspiration) on a single chip to enable cell electrical and mechanical characterization. To investigate the performance of the device design, electrical and mechanical properties of MC-3T3 osteoblast cells were measured. Based on electrical models, membrane capacitance of MC-3T3 cells was determined to be 3.39 ± 1.23 pF and 2.99 ± 0.82 pF at the aspiration pressure of 50 Pa and 100 Pa, respectively. Cytoplasm resistance values were 110.1±37.7 kω (50 Pa) and 145.2±44.3 kω (100 Pa). Aspiration length of cells was found to be 0.813± 0.351 m at 50 Pa and 1.771 ± 0.623 m at 100 Pa. Quantified Young's modulus values were 377±189 Pa at 50 Pa and 344±156 Pa at 100 Pa. Experimental results demonstrate the device's capability for characterizing both electrical and mechanical properties of single cells.
KW - Cellular Biophysics
KW - Impedance Spectroscopy
KW - Microfluidics
KW - Micropipette Aspiration
KW - Single Cell Analysis
UR - http://www.scopus.com/inward/record.url?scp=80052116315&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052116315&partnerID=8YFLogxK
U2 - 10.1109/TRANSDUCERS.2011.5969300
DO - 10.1109/TRANSDUCERS.2011.5969300
M3 - Conference contribution
AN - SCOPUS:80052116315
SN - 9781457701573
T3 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
SP - 1192
EP - 1195
BT - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
T2 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Y2 - 5 June 2011 through 9 June 2011
ER -