A method for obtaining large populations of synchronized caenorhabditis elegans dauer larvae

Maria C. Ow, Sarah E. Hall

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

The C. elegans dauer is an attractive model with which to investigate fundamental biological questions, such as how environmental cues are sensed and are translated into developmental decisions through a series of signaling cascades that ultimately result in a transformed animal. Here we describe a simple method of using egg white plates to obtain highly synchronized purifi ed dauers that can be used in downstream applications requiring large quantities of dauers or postdauer animals.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages209-219
Number of pages11
DOIs
StatePublished - 2015

Publication series

NameMethods in Molecular Biology
Volume1327
ISSN (Print)1064-3745

Keywords

  • Caenorhabditis elegans
  • Dauer
  • Diapause
  • Egg white plate
  • Pheromone
  • Postdauer
  • Stress

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Ow, M. C., & Hall, S. E. (2015). A method for obtaining large populations of synchronized caenorhabditis elegans dauer larvae. In Methods in Molecular Biology (pp. 209-219). (Methods in Molecular Biology; Vol. 1327). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-2842-2_15