A DNA repair protein and histone methyltransferase interact to promote genome stability in the Caenorhabditis elegans germ line

Bing Yang, Xia Xu, Logan Russell, Matthew T. Sullenberger, Judith L. Yanowitz, Eleanor M Maine

Research output: Contribution to journalArticle

Abstract

Histone modifications regulate gene expression and chromosomal events, yet how histone-modifying enzymes are targeted is poorly understood. Here we report that a conserved DNA repair protein, SMRC-1, associates with MET-2, the C. elegans histone methyltransferase responsible for H3K9me1 and me2 deposition. We used molecular, genetic, and biochemical methods to investigate the biological role of SMRC-1 and to explore its relationship with MET-2. SMRC-1, like its mammalian ortholog SMARCAL1, provides protection from DNA replication stress. SMRC-1 limits accumulation of DNA damage and promotes germline and embryonic viability. MET-2 and SMRC-1 localize to mitotic and meiotic germline nuclei, and SMRC-1 promotes an increase in MET-2 abundance in mitotic germline nuclei upon replication stress. In the absence of SMRC-1, germline H3K9me2 generally decreases after multiple generations at high culture temperature. Genetic data are consistent with MET-2 and SMRC-1 functioning together to limit replication stress in the germ line and in parallel to promote other germline processes. We hypothesize that loss of SMRC-1 activity causes chronic replication stress, in part because of insufficient recruitment of MET-2 to nuclei.

Original languageEnglish (US)
Pages (from-to)e1007992
JournalPLoS genetics
Volume15
Issue number2
DOIs
StatePublished - Feb 1 2019

Fingerprint

Histone Code
Protein Methyltransferases
Genomic Instability
Caenorhabditis elegans
methyltransferases
DNA repair
DNA Replication
histones
Germ Cells
DNA Repair
Histones
DNA Damage
repair
Molecular Biology
germ cells
genome
Gene Expression
DNA
Temperature
protein

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

A DNA repair protein and histone methyltransferase interact to promote genome stability in the Caenorhabditis elegans germ line. / Yang, Bing; Xu, Xia; Russell, Logan; Sullenberger, Matthew T.; Yanowitz, Judith L.; Maine, Eleanor M.

In: PLoS genetics, Vol. 15, No. 2, 01.02.2019, p. e1007992.

Research output: Contribution to journalArticle

Yang, Bing ; Xu, Xia ; Russell, Logan ; Sullenberger, Matthew T. ; Yanowitz, Judith L. ; Maine, Eleanor M. / A DNA repair protein and histone methyltransferase interact to promote genome stability in the Caenorhabditis elegans germ line. In: PLoS genetics. 2019 ; Vol. 15, No. 2. pp. e1007992.
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