A deletion in the Hermansky–Pudlak syndrome 4 (Hps4) gene appears to be responsible for albinism in channel catfish

Yueru Li, Xin Geng, Lisui Bao, Ahmed Elaswad, Kevin W. Huggins, Rex Dunham, Zhanjian "John" Liu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Albinism is caused by a series of genetic abnormalities leading to reduction of melanin production. Albinism is quite frequent in catfish, but the causative gene and the molecular basis were unknown. In this study, we conducted a genome-wide association study (GWAS) using the 250 K SNP array. The GWAS analysis allowed mapping of the albino phenotype in the Hermansky–Pudlak syndrome 4 (Hps4) gene, which is known to be involved in melanosome biosynthesis. Sequencing analysis revealed that a 99-bp deletion was present in all analyzed albino catfish at the intron 2 and exon 3 junction. This deletion led to the skipping of the entire exon 3 which was confirmed by RT-PCR. Therefore, Hps4 was determined to be the candidate gene of the catfish albinism.

Original languageEnglish (US)
Pages (from-to)663-670
Number of pages8
JournalMolecular Genetics and Genomics
Volume292
Issue number3
DOIs
StatePublished - Jun 1 2017
Externally publishedYes

Fingerprint

Albinism
Ictaluridae
Catfishes
Genome-Wide Association Study
Exons
Genes
Melanosomes
Melanins
Introns
Single Nucleotide Polymorphism
Phenotype
Polymerase Chain Reaction

Keywords

  • Albinism
  • Catfish
  • GWAS
  • Hps4
  • Melanin
  • Mutation
  • Pigmentation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

A deletion in the Hermansky–Pudlak syndrome 4 (Hps4) gene appears to be responsible for albinism in channel catfish. / Li, Yueru; Geng, Xin; Bao, Lisui; Elaswad, Ahmed; Huggins, Kevin W.; Dunham, Rex; Liu, Zhanjian "John".

In: Molecular Genetics and Genomics, Vol. 292, No. 3, 01.06.2017, p. 663-670.

Research output: Contribution to journalArticle

Li, Yueru ; Geng, Xin ; Bao, Lisui ; Elaswad, Ahmed ; Huggins, Kevin W. ; Dunham, Rex ; Liu, Zhanjian "John". / A deletion in the Hermansky–Pudlak syndrome 4 (Hps4) gene appears to be responsible for albinism in channel catfish. In: Molecular Genetics and Genomics. 2017 ; Vol. 292, No. 3. pp. 663-670.
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