@article{85d0801c800f4869b9cd609738d336d7,
title = "A combined hiPSC-derived endothelial cell and in vitro microfluidic platform for assessing biomaterial-based angiogenesis",
abstract = "Human induced pluripotent stem cell (hiPSC) derived angiogenesis models present a unique opportunity for patient-specific platforms to study the complex process of angiogenesis and the endothelial cell response to biomaterial and biophysical changes in a defined microenvironment. We present a refined method for differentiating hiPSCs into a CD31 + endothelial cell population (hiPSC-ECs) using a single basal medium from pluripotency to the final stage of differentiation. This protocol produces endothelial cells that are functionally competent in assays following purification. Subsequently, an in vitro angiogenesis model was developed by encapsulating the hiPSC-ECs into a tunable, growth factor sequestering hyaluronic acid (HyA) matrix where they formed stable, capillary-like networks that responded to environmental stimuli. Perfusion of the networks was demonstrated using fluorescent beads in a microfluidic device designed to study angiogenesis. The combination of hiPSC-ECs, bioinspired hydrogel, and the microfluidic platform creates a unique testbed for rapidly assessing the performance of angiogenic biomaterials.",
keywords = "Differentiation, Human induced pluripotent stem cells (hiPSCs), Hyaluronic acid, Hydrogel, In vitro angiogenesis model, hiPSC-derived endothelial cells (hiPSC-ECs)",
author = "Natividad-Diaz, {Sylvia L.} and Shane Browne and Jha, {Amit K.} and Zhen Ma and Samir Hossainy and Kurokawa, {Yosuke K.} and George, {Steven C.} and Healy, {Kevin E.}",
note = "Funding Information: This research was supported in part by NIH grants UH2TR000487 (K. E. H.) and UH3TR000487 (K. E. H.). Additionally, this work was supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGE 1106400 , and the Jan Fandrianto and Selfia Halim Endowed Chair (K.E.H.). Any opinion, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. S. L.N.-D. was also supported by doctoral fellowships from NIH T32 GM 098218 and the Siebel Scholars Foundation . We acknowledge and thank the U.C. Berkeley Stem Cell Center, Flow Cytometry Core Facilities at U.C. Berkeley, Mary West, Hector Nolla, and Brian Siemens for their assistance with this work. Funding Information: This research was supported in part by NIH grants UH2TR000487 (K. E. H.) and UH3TR000487 (K. E. H.). Additionally, this work was supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGE 1106400, and the Jan Fandrianto and Selfia Halim Endowed Chair (K.E.H.). Any opinion, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. S. L.N.-D. was also supported by doctoral fellowships from NIH T32 GM 098218 and the Siebel Scholars Foundation. We acknowledge and thank the U.C. Berkeley Stem Cell Center, Flow Cytometry Core Facilities at U.C. Berkeley, Mary West, Hector Nolla, and Brian Siemens for their assistance with this work. Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",
year = "2019",
month = feb,
doi = "10.1016/j.biomaterials.2018.11.032",
language = "English (US)",
volume = "194",
pages = "73--83",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier",
}