TY - JOUR
T1 - A cholesterol-binding and transporting protein from rat liver mitochondria
AU - Campbell, Andrew M.
AU - Capuano, Aaron
AU - Chan, Samuel H.P.
N1 - Funding Information:
This research was supported by National Institute of Health Grant CA-20454. We thank B.W.C. Lau and M. Weyant for technical assistance.
PY - 2002/12/23
Y1 - 2002/12/23
N2 - In this communication, we present results indicating a protein isolated from rat liver mitochondrial intermembrane space that is capable of binding cholesterol and transporting it between the inner and outer mitochondrial membranes. This protein has a molecular weight of 57.5 kDa by SDS-PAGE; however, under native conditions, there is cholesterol-binding capability only as a 115 kDa dimer. Our data show that this dimeric protein may play a role in the regulation of mitochondrial membrane cholesterol levels, a perquisite for the optimal activity of inner mitochondrial membrane-associated enzyme complexes. In addition, it appears that this protein is largely responsible for the differences in membrane cholesterol levels observed in normal and hepatoma mitochondria, a discrepancy which may help to explain the lack of energy production via oxidative phosporylation in malignant tumor mitochondria.
AB - In this communication, we present results indicating a protein isolated from rat liver mitochondrial intermembrane space that is capable of binding cholesterol and transporting it between the inner and outer mitochondrial membranes. This protein has a molecular weight of 57.5 kDa by SDS-PAGE; however, under native conditions, there is cholesterol-binding capability only as a 115 kDa dimer. Our data show that this dimeric protein may play a role in the regulation of mitochondrial membrane cholesterol levels, a perquisite for the optimal activity of inner mitochondrial membrane-associated enzyme complexes. In addition, it appears that this protein is largely responsible for the differences in membrane cholesterol levels observed in normal and hepatoma mitochondria, a discrepancy which may help to explain the lack of energy production via oxidative phosporylation in malignant tumor mitochondria.
KW - Adenine nucleotide transporter
KW - Cholesterol-binding protein
KW - Mitochondrial membrane
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U2 - 10.1016/S0005-2736(02)00607-7
DO - 10.1016/S0005-2736(02)00607-7
M3 - Article
C2 - 12488045
AN - SCOPUS:0037164704
SN - 0005-2736
VL - 1567
SP - 123
EP - 132
JO - Biochimica et Biophysica Acta-Biomembranes
JF - Biochimica et Biophysica Acta-Biomembranes
ER -