A chemist's view of inflammation in contusion injured spinal cord in a rat model

Noninvasive, noncontact, in vivo Raman spectroscopy minutes to hours after injury

Seth Fillioe, Kyle Kelly Bishop, Sai Han Tun, Paul Dent, Bin Deng, Charles M. Peterson, Jerry Goodisman, Julie M Hasenwinkel, Joseph Chaiken

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The initial biological response to spinal cord injury is initiated by intra-and extracellular chemical signals. We compare Raman spectra of injured spinal cord obtained minutes after injury to those of uninjured spinal cord to obtain chemical information that precedes the biological response. We studied 29 rats including both Injured and Control using Raman spectra of spinal cords in vivo. Principal Component Analysis (PCA) indicates that <99% of the variation of these spectra across both Injured and Control groups is accounted for with 3 components. The first component does not vary significantly representing structural materials. The second and third components reflect the variation in the chemistry of the cerebrospinal fluid. We demonstrate the first noninvasive in vivo measurement of pH in the CSF using only Raman spectra. We hypothesize that the earliest inflammatory response to mild contusive injury reflects the chemistry of inorganic phosphate present at abnormally high concentrations, likely due to physical disruption of the blood-brain barrier in the choroid plexus and/or mitochondrial release of phosphate, reacting with CSF water.

Original languageEnglish (US)
Title of host publicationPhotonic Diagnosis and Treatment of Infections and Inflammatory Diseases II
EditorsTianhong Dai, Jurgen Popp, Mei X. Wu
PublisherSPIE
ISBN (Electronic)9781510623682
DOIs
StatePublished - Jan 1 2019
EventPhotonic Diagnosis and Treatment of Infections and Inflammatory Diseases II 2019 - San Francisco, United States
Duration: Feb 4 2019Feb 5 2019

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume10863
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferencePhotonic Diagnosis and Treatment of Infections and Inflammatory Diseases II 2019
CountryUnited States
CitySan Francisco
Period2/4/192/5/19

Fingerprint

spinal cord
Inflammation
Raman Spectra
Raman Spectroscopy
Non-contact
rats
Raman spectroscopy
Rats
Raman spectra
Phosphate
Raman scattering
Chemistry
phosphates
spinal cord injuries
chemistry
cerebrospinal fluid
blood-brain barrier
Phosphates
principal components analysis
Cerebrospinal fluid

Keywords

  • Cerebrospinal fluid
  • In vivo
  • Inflammation
  • Noninvasive
  • PH
  • Phosphate
  • PV[O]H
  • Raman
  • Spinal cord injury

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Fillioe, S., Bishop, K. K., Tun, S. H., Dent, P., Deng, B., Peterson, C. M., ... Chaiken, J. (2019). A chemist's view of inflammation in contusion injured spinal cord in a rat model: Noninvasive, noncontact, in vivo Raman spectroscopy minutes to hours after injury. In T. Dai, J. Popp, & M. X. Wu (Eds.), Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II [108630G] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10863). SPIE. https://doi.org/10.1117/12.2509082

A chemist's view of inflammation in contusion injured spinal cord in a rat model : Noninvasive, noncontact, in vivo Raman spectroscopy minutes to hours after injury. / Fillioe, Seth; Bishop, Kyle Kelly; Tun, Sai Han; Dent, Paul; Deng, Bin; Peterson, Charles M.; Goodisman, Jerry; Hasenwinkel, Julie M; Chaiken, Joseph.

Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II. ed. / Tianhong Dai; Jurgen Popp; Mei X. Wu. SPIE, 2019. 108630G (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10863).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fillioe, S, Bishop, KK, Tun, SH, Dent, P, Deng, B, Peterson, CM, Goodisman, J, Hasenwinkel, JM & Chaiken, J 2019, A chemist's view of inflammation in contusion injured spinal cord in a rat model: Noninvasive, noncontact, in vivo Raman spectroscopy minutes to hours after injury. in T Dai, J Popp & MX Wu (eds), Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II., 108630G, Proceedings of SPIE - The International Society for Optical Engineering, vol. 10863, SPIE, Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II 2019, San Francisco, United States, 2/4/19. https://doi.org/10.1117/12.2509082
Fillioe S, Bishop KK, Tun SH, Dent P, Deng B, Peterson CM et al. A chemist's view of inflammation in contusion injured spinal cord in a rat model: Noninvasive, noncontact, in vivo Raman spectroscopy minutes to hours after injury. In Dai T, Popp J, Wu MX, editors, Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II. SPIE. 2019. 108630G. (Proceedings of SPIE - The International Society for Optical Engineering). https://doi.org/10.1117/12.2509082
Fillioe, Seth ; Bishop, Kyle Kelly ; Tun, Sai Han ; Dent, Paul ; Deng, Bin ; Peterson, Charles M. ; Goodisman, Jerry ; Hasenwinkel, Julie M ; Chaiken, Joseph. / A chemist's view of inflammation in contusion injured spinal cord in a rat model : Noninvasive, noncontact, in vivo Raman spectroscopy minutes to hours after injury. Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II. editor / Tianhong Dai ; Jurgen Popp ; Mei X. Wu. SPIE, 2019. (Proceedings of SPIE - The International Society for Optical Engineering).
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