A cDNA microarray for Crassostrea virginica and C. gigas

Matthew J. Jenny, Robert W. Chapman, Annalaura Mancia, Yian A. Chen, David J. McKillen, Hal Trent, Paul Lang, Jean Michel Escoubas, Evelyne Bachere, Viviane Boulo, Z. John Liu, Paul S. Gross, Charles Cunningham, Pauline M. Cupit, Arnaud Tanguy, Ximing Guo, Dario Moraga, Isabelle Boutet, Arnaud Huvet, Sylvain De GuiseJonas S. Almeida, Gregory W. Warr

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

The eastern oyster, Crassostrea virginica, and the Pacific oyster, C. gigas, are species of global economic significance as well as important components of estuarine ecosystems and models for genetic and environmental studies. To enhance the molecular tools available for oyster research, an international group of collaborators has constructed a 27,496-feature cDNA microarray containing 4460 sequences derived from C. virginica, 2320 from C. gigas, and 16 non-oyster DNAs serving as positive and negative controls. The performance of the array was assessed by gene expression profiling using gill and digestive gland RNA derived from both C. gigas and C. virginica, and digestive gland RNA from C. ariakensis. The utility of the microarray for detection of homologous genes by cross-hybridization between species was also assessed and the correlation between hybridization intensity and sequence homology for selected genes determined. The oyster cDNA microarray is publicly available to the research community on a cost-recovery basis.

Original languageEnglish (US)
Pages (from-to)577-591
Number of pages15
JournalMarine Biotechnology
Volume9
Issue number5
DOIs
StatePublished - Oct 1 2007
Externally publishedYes

Keywords

  • Bivalve
  • Crassostrea gigas
  • Crassostrea virginica
  • Heterologous hybridization
  • Microarray
  • Oyster

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science

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  • Cite this

    Jenny, M. J., Chapman, R. W., Mancia, A., Chen, Y. A., McKillen, D. J., Trent, H., Lang, P., Escoubas, J. M., Bachere, E., Boulo, V., Liu, Z. J., Gross, P. S., Cunningham, C., Cupit, P. M., Tanguy, A., Guo, X., Moraga, D., Boutet, I., Huvet, A., ... Warr, G. W. (2007). A cDNA microarray for Crassostrea virginica and C. gigas. Marine Biotechnology, 9(5), 577-591. https://doi.org/10.1007/s10126-007-9041-1