A cDNA microarray for Crassostrea virginica and C. gigas

Matthew J. Jenny, Robert W. Chapman, Annalaura Mancia, Yian A. Chen, David J. McKillen, Hal Trent, Paul Lang, Jean Michel Escoubas, Evelyne Bachere, Viviane Boulo, Zhanjian "John" Liu, Paul S. Gross, Charles Cunningham, Pauline M. Cupit, Arnaud Tanguy, Ximing Guo, Dario Moraga, Isabelle Boutet, Arnaud Huvet, Sylvain De GuiseJonas S. Almeida, Gregory W. Warr

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The eastern oyster, Crassostrea virginica, and the Pacific oyster, C. gigas, are species of global economic significance as well as important components of estuarine ecosystems and models for genetic and environmental studies. To enhance the molecular tools available for oyster research, an international group of collaborators has constructed a 27,496-feature cDNA microarray containing 4460 sequences derived from C. virginica, 2320 from C. gigas, and 16 non-oyster DNAs serving as positive and negative controls. The performance of the array was assessed by gene expression profiling using gill and digestive gland RNA derived from both C. gigas and C. virginica, and digestive gland RNA from C. ariakensis. The utility of the microarray for detection of homologous genes by cross-hybridization between species was also assessed and the correlation between hybridization intensity and sequence homology for selected genes determined. The oyster cDNA microarray is publicly available to the research community on a cost-recovery basis.

Original languageEnglish (US)
Pages (from-to)577-591
Number of pages15
JournalMarine Biotechnology
Volume9
Issue number5
DOIs
StatePublished - Oct 2007
Externally publishedYes

Fingerprint

Crassostrea
Crassostrea virginica
Crassostrea gigas
Oligonucleotide Array Sequence Analysis
Ostreidae
RNA
estuarine ecosystem
gene
homology
oysters
gene expression
hybridization
DNA
Genetic Models
Gene Expression Profiling
Sequence Homology
sequence homology
economics
Research
Genes

Keywords

  • Bivalve
  • Crassostrea gigas
  • Crassostrea virginica
  • Heterologous hybridization
  • Microarray
  • Oyster

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science

Cite this

Jenny, M. J., Chapman, R. W., Mancia, A., Chen, Y. A., McKillen, D. J., Trent, H., ... Warr, G. W. (2007). A cDNA microarray for Crassostrea virginica and C. gigas. Marine Biotechnology, 9(5), 577-591. https://doi.org/10.1007/s10126-007-9041-1

A cDNA microarray for Crassostrea virginica and C. gigas. / Jenny, Matthew J.; Chapman, Robert W.; Mancia, Annalaura; Chen, Yian A.; McKillen, David J.; Trent, Hal; Lang, Paul; Escoubas, Jean Michel; Bachere, Evelyne; Boulo, Viviane; Liu, Zhanjian "John"; Gross, Paul S.; Cunningham, Charles; Cupit, Pauline M.; Tanguy, Arnaud; Guo, Ximing; Moraga, Dario; Boutet, Isabelle; Huvet, Arnaud; De Guise, Sylvain; Almeida, Jonas S.; Warr, Gregory W.

In: Marine Biotechnology, Vol. 9, No. 5, 10.2007, p. 577-591.

Research output: Contribution to journalArticle

Jenny, MJ, Chapman, RW, Mancia, A, Chen, YA, McKillen, DJ, Trent, H, Lang, P, Escoubas, JM, Bachere, E, Boulo, V, Liu, ZJ, Gross, PS, Cunningham, C, Cupit, PM, Tanguy, A, Guo, X, Moraga, D, Boutet, I, Huvet, A, De Guise, S, Almeida, JS & Warr, GW 2007, 'A cDNA microarray for Crassostrea virginica and C. gigas', Marine Biotechnology, vol. 9, no. 5, pp. 577-591. https://doi.org/10.1007/s10126-007-9041-1
Jenny MJ, Chapman RW, Mancia A, Chen YA, McKillen DJ, Trent H et al. A cDNA microarray for Crassostrea virginica and C. gigas. Marine Biotechnology. 2007 Oct;9(5):577-591. https://doi.org/10.1007/s10126-007-9041-1
Jenny, Matthew J. ; Chapman, Robert W. ; Mancia, Annalaura ; Chen, Yian A. ; McKillen, David J. ; Trent, Hal ; Lang, Paul ; Escoubas, Jean Michel ; Bachere, Evelyne ; Boulo, Viviane ; Liu, Zhanjian "John" ; Gross, Paul S. ; Cunningham, Charles ; Cupit, Pauline M. ; Tanguy, Arnaud ; Guo, Ximing ; Moraga, Dario ; Boutet, Isabelle ; Huvet, Arnaud ; De Guise, Sylvain ; Almeida, Jonas S. ; Warr, Gregory W. / A cDNA microarray for Crassostrea virginica and C. gigas. In: Marine Biotechnology. 2007 ; Vol. 9, No. 5. pp. 577-591.
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