A cDNA microarray for Crassostrea virginica and C. gigas

Matthew J. Jenny; Robert W. Chapman; Annalaura Mancia; Yian A. Chen; David J. McKillen; Hal Trent; Paul Lang; Jean Michel Escoubas; Evelyne Bachere; Viviane Boulo; Z. John Liu; Paul S. Gross; Charles Cunningham; Pauline M. Cupit; Arnaud Tanguy; Ximing Guo; Dario Moraga; Isabelle Boutet; Arnaud Huvet; Sylvain De Guise; Jonas S. Almeida; Gregory W. Warr

doi:10.1007/s10126-007-9041-1

A cDNA microarray for Crassostrea virginica and C. gigas

Matthew J. Jenny, Robert W. Chapman, Annalaura Mancia, Yian A. Chen, David J. McKillen, Hal Trent, Paul Lang, Jean Michel Escoubas, Evelyne Bachere, Viviane Boulo, Z. John Liu, Paul S. Gross, Charles Cunningham, Pauline M. Cupit, Arnaud Tanguy, Ximing Guo, Dario Moraga, Isabelle Boutet, Arnaud Huvet, Sylvain De GuiseJonas S. Almeida, Gregory W. Warr

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

The eastern oyster, Crassostrea virginica, and the Pacific oyster, C. gigas, are species of global economic significance as well as important components of estuarine ecosystems and models for genetic and environmental studies. To enhance the molecular tools available for oyster research, an international group of collaborators has constructed a 27,496-feature cDNA microarray containing 4460 sequences derived from C. virginica, 2320 from C. gigas, and 16 non-oyster DNAs serving as positive and negative controls. The performance of the array was assessed by gene expression profiling using gill and digestive gland RNA derived from both C. gigas and C. virginica, and digestive gland RNA from C. ariakensis. The utility of the microarray for detection of homologous genes by cross-hybridization between species was also assessed and the correlation between hybridization intensity and sequence homology for selected genes determined. The oyster cDNA microarray is publicly available to the research community on a cost-recovery basis.

Original language	English (US)
Pages (from-to)	577-591
Number of pages	15
Journal	Marine Biotechnology
Volume	9
Issue number	5
DOIs	https://doi.org/10.1007/s10126-007-9041-1
State	Published - Oct 2007
Externally published	Yes

Keywords

Bivalve
Crassostrea gigas
Crassostrea virginica
Heterologous hybridization
Microarray
Oyster

ASJC Scopus subject areas

Biotechnology
Aquatic Science

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Jenny, M. J., Chapman, R. W., Mancia, A., Chen, Y. A., McKillen, D. J., Trent, H., Lang, P., Escoubas, J. M., Bachere, E., Boulo, V., Liu, Z. J., Gross, P. S., Cunningham, C., Cupit, P. M., Tanguy, A., Guo, X., Moraga, D., Boutet, I., Huvet, A., ... Warr, G. W. (2007). A cDNA microarray for Crassostrea virginica and C. gigas. Marine Biotechnology, 9(5), 577-591. https://doi.org/10.1007/s10126-007-9041-1

Jenny, MJ, Chapman, RW, Mancia, A, Chen, YA, McKillen, DJ, Trent, H, Lang, P, Escoubas, JM, Bachere, E, Boulo, V, Liu, ZJ, Gross, PS, Cunningham, C, Cupit, PM, Tanguy, A, Guo, X, Moraga, D, Boutet, I, Huvet, A, De Guise, S, Almeida, JS & Warr, GW 2007, 'A cDNA microarray for Crassostrea virginica and C. gigas', Marine Biotechnology, vol. 9, no. 5, pp. 577-591. https://doi.org/10.1007/s10126-007-9041-1

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abstract = "The eastern oyster, Crassostrea virginica, and the Pacific oyster, C. gigas, are species of global economic significance as well as important components of estuarine ecosystems and models for genetic and environmental studies. To enhance the molecular tools available for oyster research, an international group of collaborators has constructed a 27,496-feature cDNA microarray containing 4460 sequences derived from C. virginica, 2320 from C. gigas, and 16 non-oyster DNAs serving as positive and negative controls. The performance of the array was assessed by gene expression profiling using gill and digestive gland RNA derived from both C. gigas and C. virginica, and digestive gland RNA from C. ariakensis. The utility of the microarray for detection of homologous genes by cross-hybridization between species was also assessed and the correlation between hybridization intensity and sequence homology for selected genes determined. The oyster cDNA microarray is publicly available to the research community on a cost-recovery basis.",

keywords = "Bivalve, Crassostrea gigas, Crassostrea virginica, Heterologous hybridization, Microarray, Oyster",

author = "Jenny, {Matthew J.} and Chapman, {Robert W.} and Annalaura Mancia and Chen, {Yian A.} and McKillen, {David J.} and Hal Trent and Paul Lang and Escoubas, {Jean Michel} and Evelyne Bachere and Viviane Boulo and Liu, {Z. John} and Gross, {Paul S.} and Charles Cunningham and Cupit, {Pauline M.} and Arnaud Tanguy and Ximing Guo and Dario Moraga and Isabelle Boutet and Arnaud Huvet and {De Guise}, Sylvain and Almeida, {Jonas S.} and Warr, {Gregory W.}",

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AU - Trent, Hal

AU - Lang, Paul

AU - Escoubas, Jean Michel

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AU - Liu, Z. John

AU - Gross, Paul S.

AU - Cunningham, Charles

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AU - Guo, Ximing

AU - Moraga, Dario

AU - Boutet, Isabelle

AU - Huvet, Arnaud

AU - De Guise, Sylvain

AU - Almeida, Jonas S.

AU - Warr, Gregory W.

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N2 - The eastern oyster, Crassostrea virginica, and the Pacific oyster, C. gigas, are species of global economic significance as well as important components of estuarine ecosystems and models for genetic and environmental studies. To enhance the molecular tools available for oyster research, an international group of collaborators has constructed a 27,496-feature cDNA microarray containing 4460 sequences derived from C. virginica, 2320 from C. gigas, and 16 non-oyster DNAs serving as positive and negative controls. The performance of the array was assessed by gene expression profiling using gill and digestive gland RNA derived from both C. gigas and C. virginica, and digestive gland RNA from C. ariakensis. The utility of the microarray for detection of homologous genes by cross-hybridization between species was also assessed and the correlation between hybridization intensity and sequence homology for selected genes determined. The oyster cDNA microarray is publicly available to the research community on a cost-recovery basis.

AB - The eastern oyster, Crassostrea virginica, and the Pacific oyster, C. gigas, are species of global economic significance as well as important components of estuarine ecosystems and models for genetic and environmental studies. To enhance the molecular tools available for oyster research, an international group of collaborators has constructed a 27,496-feature cDNA microarray containing 4460 sequences derived from C. virginica, 2320 from C. gigas, and 16 non-oyster DNAs serving as positive and negative controls. The performance of the array was assessed by gene expression profiling using gill and digestive gland RNA derived from both C. gigas and C. virginica, and digestive gland RNA from C. ariakensis. The utility of the microarray for detection of homologous genes by cross-hybridization between species was also assessed and the correlation between hybridization intensity and sequence homology for selected genes determined. The oyster cDNA microarray is publicly available to the research community on a cost-recovery basis.

KW - Bivalve

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KW - Crassostrea virginica

KW - Heterologous hybridization

KW - Microarray

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A cDNA microarray for Crassostrea virginica and C. gigas

Abstract

Keywords

ASJC Scopus subject areas

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