AN AUTOMATED SINGLE CELL SEPARATION TECHNIQUE TO IMPROVE MIXTURE DECONVOLUTION

Marciano, M. (Speaker)

Activity: Talk typesOral presentation

Description

This webinar will provide an evaluation of the DEPArray™, an instrument that can be used to identify, separate, and recover single cells or groups of cells prior to genetic analysis, thereby resolving challenges inherent to mixed biological evidence. Isolation of cells is performed using a single-use micro-fluidic cartridge containing an array of di-electrophoretic (DEP) cages (electrodes) that can be individually controlled permitting the capture and manipulation of single or groups of cells. Once captured, the cell(s) can then be routed to a recovery chamber by changing the electric field pattern and subsequently collected in a standard micro-centrifuge tube. The DEPArray™ has been used in the cancer research community since 2010 to primarily identify and isolate circulating tumor cells from large populations of white blood cells. Once this small population of target cells has been isolated from the non-target population, analyses such as PCR and whole genome sequencing can be used to characterize the sample in the absence of the “noise” created by non-target cells. This problem set largely mirrors those experienced in forensic DNA analyses, where samples are commonly composed of more than one donor of the same or differing cell types in differing quantities.

PeriodNov 2020
Held atNational Institute of Justice/Department of Justice
Degree of RecognitionNational

Keywords

  • forensic DNA